Liquid Culture Starter Pack

• 2 x syringes
• 2 x needles
• 2 x MEA plates
• 2 x pieces of parafilm
• 2 x Swabs

This is enough to make 1lt of liquid Culture.

To correctly use this product you should be working with at least a Still Air Box or Laminar Flow Hood. You will need a modified jar to grow this culture in, if you dont have one you can purchase ours from here. Step-bu-step information can be found below.

Directions for use:

1. Mix the contents with 1l of bottled water. Use a blender / whisk / fork to blend together well
2. Add contents to modified jar, we recommend 500l per 1l jar so as not to overfill.
3. Add a marble or broken glass into the jat to healp break up the mycelium
4. Tighten the jar lid then loosen by 90degrees so the pressure can escape from the lid rather than through the syringe filter.
5. Wrap tin foil over the lid
6. Cook for 22 minutes at 15psi / 121c
7. You must let the pressure release slowly or the LC will boil over.
8. Remove from pressure cooker and tighten lid
9. When cool inoculate with LC syringe
10. Swirl the LC each day to try break apart the growing mycelium, try not get the filter wet each time you do this.
11. After about 3 weeks it should be ready
12. Test out the Liquid Culture on the MEA plates provided by withdrawing some from the bottle using the syringe and adding a few drops to the plate ( about 0.25 ml ) wrap the MEA plate with parafilm after.
13. You should see healthy white mycelium on the plate within 1 week

Tips on using LC:

1. Try keep the filter dry as much as possible.
2. Wipe the injection port with alcohol before each extraction, I like to wipe down the whole bottle before using each time.
3. Don't overfill the bottle or you will get the filter wet each time you use it.
4. Do not tighten lid before cooking, the changes in pressure can break the syringe filter