Totius was responsible for much of the translation of the Bible into Afrikaans, finishing what his father Stephanus Jacobus du Toit had begun. He also put a huge amount of work into producing poetic versions of the Psalms in Afrikaans. His poetry was in the main lyrical and dealt, inter alia, with faith and with nature, as well as more political themes such as British imperialism and the Afrikaner nation. He left behind many collections of poems, including Trekkerswee (1915; Trekkers' Grief), Passieblomme (1934; Passion Flowers), "Uit donker Afrika" (1936; "From deep inside Africa") as well as a volume of poetry rhymed Psalms were published.

He was on the committee that founded Potchefstroom Gimnasium in 1907 and chancellor of the Potchefstroom University for Christian Higher Education, from 1951-1953.